SubtiBank SubtiBank
amhX [2018-02-11T23:01:13.000Z]
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amhX [2018-02-11T23:01:13.000Z]

amidohydrolase
locus
BSU03010
pI
6.12
mw
42.37 kDa
protein length
389 aa Sequence Blast
gene length
1167 bp Sequence Blast
function
unknown
product
amidohydrolase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

    • Gene

    Coordinates
    324,038 → 325,189

    The protein

    Protein family

  • peptidase M20 family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [gene|C99AA3FDBB561BCED60915078DC746690C071753|sndB](32%)

    • Structure

  • [PDB|1YSJ] (from ''Bacillus subtilis'' (32%) )

    • Expression and Regulation

    Operons

    genes
    [gene|AE824A62089631F129C3AA469B6ACA71DC13D2F8|amhX]
    description
    [Pubmed|8768514]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8768514], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|24843172], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
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    Biological materials

    Mutant

  • BKE03010 (Δ[gene|AE824A62089631F129C3AA469B6ACA71DC13D2F8|amhX]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTAAGAAATACCTCCT, downstream forward: _UP4_TAACGCAAAAAAAGCAGCCT
  • BKK03010 (Δ[gene|AE824A62089631F129C3AA469B6ACA71DC13D2F8|amhX]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTAAGAAATACCTCCT, downstream forward: _UP4_TAACGCAAAAAAAGCAGCCT

    • References

  • 8768514,24843172